0000117: Spectrophotometric measurement for cell density

ID	Project	Category	View Status	Date Submitted	Last Update

0000117	jFermi	Peripheral	public	2022-12-11 15:46	2022-12-11 15:46

Reporter	acet	Assigned To
Priority	low	Severity	feature	Reproducibility	N/A
Status	new	Resolution	open
Product Version	6.4

Summary	0000117: Spectrophotometric measurement for cell density
Description	The spectrophotometer provides a “quick and dirty” way to estimate the density of cells in a culture. In contrast to spot plates, which must be incubated for several days before colonies appear, spectrophotometer readings can be instantly converted into cell densities. On the other hand, the method does not discriminate between living and dead cells. The spectrophotometric method is based on light scattering by cells in the culture. When the light in a spectrophotometer hits a large particle such as a cell, light rays are deflected from a straight path and these light rays may not reach the detector. The greater the number of cells in a sample, the more light scattering occurs. The light scattering ability of a cell depends on its size and geometry, so a calibration curve is necessary to extrapolate optical density measurements to cell number. https://github.com/publiclab/spectral-workbench.js + SMA-E Spectrometer
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Date Modified	Username	Field	Change
2022-12-11 15:46	acet	New Issue